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The Orchid Seedbank Project
PO Box 7042
Chandler, AZ 85246

bridgen

Bridgen (Bridgen, 1986)

Originally cited by Bridgen, 1986. Do-it-yourself cloning. Greenhouse  Grower 1986 (May): 43-47, in Kyte and Kleyn, 1996. Plants from test tubes, 3rd edition. Timber Press, Portland, OR.

    Table sugar: 30 cc (1/8 cup)
    Tap water: 237 cc (1 cup)
    Nutrient solution from stock solution containing 1.25 cc (1/4 tsp) all-purpose 10-10-10 fertilizer in 4 liters (1 gallon) of water 118 cc: (1/2 cup)
    Inositol tablet (250 mg): 1/4 tablet
    Vitamin tablet with thiamine: 1/4 tablet
    Agar flakes: 30 cc (2 tablespoons)
    Combine contents in a flask, boil while stirring. Dispense into  culture vessels, and autoclave.

Source: Hicks, A. Asymbiotic Technique of Orchid Seed Germination. Orchid Seedbank Project, Socorro, New Mexico. 1999.

 

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The Orchid Seedbank Project
PO Box 7042
Chandler, AZ 85246