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Bridgen (Bridgen, 1986)
Originally cited by Bridgen, 1986. Do-it-yourself cloning. Greenhouse Grower 1986 (May): 43-47, in Kyte and Kleyn, 1996. Plants from test tubes, 3rd edition. Timber Press, Portland, OR.
- Table sugar: 30 cc (1/8 cup)
- Tap water: 237 cc (1 cup)
- Nutrient solution from stock solution containing 1.25 cc (1/4 tsp) all-purpose 10-10-10 fertilizer in 4 liters (1 gallon) of water 118 cc: (1/2 cup)
- Inositol tablet (250 mg): 1/4 tablet
- Vitamin tablet with thiamine: 1/4 tablet
- Agar flakes: 30 cc (2 tablespoons)
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Combine contents in a flask, boil while stirring. Dispense into culture vessels, and autoclave.
Source: Hicks, A. Asymbiotic Technique of Orchid Seed Germination. Orchid Seedbank Project, Socorro, New Mexico. 1999.
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