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Clements’ isolation medium
Originally cited by Clements, 1982. Developments in the symbiotic germination of Australian terrestrial orchids. In Proceedings of the 10th World Orchid Conference 1981, ed. J. Stewart and C.N. Van der Merwe, pp. 269-273. Johannesburg: South African Orchid Council.
Used for isolation of Australian orchid fungi.
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NaNO3
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300 mg
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KH2PO4
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200 mg
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MgSO4 * 7 H2O
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100 mg
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KCl
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100 mg
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Streptomycin sulphate
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50 mg
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Sucrose
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5 g
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Yeast extract
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100 mg
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Agar
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10 g
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Adjust pH to between 4.5 and 5.0.
Source: Rasmussen, H. Terrestrial orchids from seed to mycotrophic plant. Cambridge University Press, Cambridge. 1995.
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