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CM1
Originally cited by Rasmussen et al., 1990. Germination and growth of mycorrhizal seedlings of Tipularia discolor (Orchidaceae) on woody debris. American Journal of Botany, Suppl., 79, 68. Modified with the addition of novobiocin from Clements et al., 1986. A preliminary report on the symbiotic germination of European terrestrial orchids. Kew Bulletin, 41, 437-45.
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Ca(NO3) 2 * 4 H2O
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200 mg
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KH2PO4
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200 mg
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MgSO4 * 7 H2O
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100 mg
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KCl
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100 mg
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Novobiocin (a heat-stable antibiotic)
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50 mg
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Sucrose
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4 g
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Yeast extract
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100 mg
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Agar
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12 g
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Medium is adjusted to pH 7.0, or 5.8 for American fungi.
Source: Rasmussen, H. Terrestrial orchids from seed to mycotrophic plant. Cambridge University Press, Cambridge. 1995.
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