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Sowing Media for Dactylorhiza species
This media may be used for the germination of Dactylorhiza species. It is very much like 1/5 strength M&S medium.
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CaCl2
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66 mg
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KH2PO4
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34 mg
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KNO3
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380 mg
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MgSO4 * 7H2O
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74 mg
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NH4NO3
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330 mg
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NaFe EDTA
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25 mg
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MS microelements
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2 mL
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MS vitamins
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1/2 mL
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Yeast extract
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1 gram
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Sugar
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20 grams
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Agar
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6 grams
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Activated charcoal
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0.5 grams
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The pH is to be adjusted to 5.8.
For replate, add 500 mg of yeast extract, and 50 grams of banana to each liter of media.
Source: R.J.A.H. Meijer, originally from J. Wetsteyn.
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