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H4 Oat Medium
Originally cited by Clements et al., 1986 (A preliminary report on the symbiotic germination of European terrestrial orchids. Kew Bulletin, 41, 437-45), modified by Rasmussen et al, 1990 (Temperature sensitivity of in vitro germination and seedling development of Dactylorhiza majalis (Orchidaceae) with and without a mycorrhizal fungus. Plant, Cell and Environment, 13, 171-7.)
For symbiotic cultures.
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NH4NO3 * 4 H2O
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72 mg
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KCl
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100 mg
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KH2PO4
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200 mg
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MgSO4 * 7 H2O
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100 mg
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Yeast extract (Difco)
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100 mg
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Glucose
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2 g
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Finely ground oats
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3 g
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Agar
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12 g
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Source: Rasmussen, H. Terrestrial orchids from seed to mycotrophic plant. Cambridge University Press, Cambridge. 1995.
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