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Hyponex Medium (Nishimura, 1982)
Originally cited by Nishimura, 1982. Japanese orchids. In Orchid Biology II: Reviews and Perspectives. Orchid seed germination and seedling culture- a manual, ed. J. Arditti, pp. 331-46. Ithaca, New York: Cornell University Press. Bottom four formulae from S. Ichihashi, as per references.
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Hyponex (commercial fertilizer)
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3 g
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Sucrose
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20, 30, or 35 g
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Peptone or tryptone
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2 g
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Activated charcoal
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2 g
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Agar
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8, 10, 12, or 15 g
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Hyponex (commercial fertilizer)
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3 g
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Sucrose
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35 g
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Agar
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15 g
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Hyponex (commercial fertilizer)
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3 g
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Sucrose
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35 g
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Apple juice
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10-20% of original volume
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Agar
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15 g
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Hyponex (commercial fertilizer)
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3 g
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Sucrose
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35 g
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Tryptone
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2 g
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Agar
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15 g
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Hyponex (commercial fertilizer)
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3 g
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Sucrose
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35 g
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Peptone or tryptone
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2 g
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Agar
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8, 10, 12, or 15 g
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Sources:
Ichihasi, S. 1968. Major medium used for orchid propagation. In: Pictoral guide of orchid biotechnology (Zukai Ran no Bio Gijutsu). Seibundo Shinkosya, Tokyo, Japan.
Rasmussen, H. Terrestrial orchids from seed to mycotrophic plant. Cambridge University Press, Cambridge. 1995.
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