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Knudson B
Originally cited by Knudson, 1925 (Physiological studies of the symbiotic germination of orchid seeds. Botanical Gazette, 79, 345-79), 1946 (A new nutrient solution for orchid seed germination. American Orchid Society Bulletin, 15, 214-17).
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Ca(NO3)2 * 4 H2O
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1000 mg
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KH2PO4
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250 mg
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MgSO4 * 7 H2O
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250 mg
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(NH4)2SO4
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500 mg
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FePO4 * 4 H2O
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50 mg (1925 formula)
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Sucrose
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20 g
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Agar
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12 g (1925 formula) 15 (1946 formula)
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Note: This medium does not employ the use of chelated iron, resulting in the formation of insoluble (and therefore unavailable) iron phosphate. As a result, this medium is very iron-poor.
Source: Rasmussen, H. Terrestrial orchids from seed to mycotrophic plant. Cambridge University Press, Cambridge. 1995.
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