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The Orchid Seedbank Project
PO Box 7042
Chandler, AZ 85246

knudsonb

Knudson B

Originally cited by Knudson, 1925 (Physiological studies of the  symbiotic germination of orchid seeds. Botanical Gazette, 79,  345-79), 1946 (A new nutrient solution for orchid seed germination. American Orchid Society Bulletin, 15, 214-17). 

 Ca(NO3)2 * 4 H2O

 1000 mg

 KH2PO4

 250 mg

 MgSO4 * 7 H2O

 250 mg

 (NH4)2SO4

 500 mg

 FePO4 * 4 H2O

 50 mg (1925 formula)

 Sucrose

 20 g

 Agar

 12 g (1925 formula)
 15 (1946 formula)

Note: This medium does not employ the use of chelated iron,  resulting in the formation of insoluble (and therefore unavailable)  iron phosphate. As a result, this medium is very iron-poor.

Source: Rasmussen, H. Terrestrial orchids from seed to mycotrophic plant. Cambridge University Press, Cambridge. 1995.

 

[OSP] [Technical Data]

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The Orchid Seedbank Project
PO Box 7042
Chandler, AZ 85246