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The Orchid Seedbank Project
PO Box 7042
Chandler, AZ 85246

knudsoncanderson

Knudson C (Anderson, 1990)

Originally cited by Anderson, 1990. Asymbiotic germination of seeds  of some North American orchids. In North American native terrestrial orchid propagation and production, ed. C.E. Sawyers, pp. 75-80. Chadds Ford, Pennsylvania: Brandywine Conservancy.

 Ca(NO3)2 * 4 H2O

 1000 mg

 KH2PO4

 228 mg

 K2HPO4

 7.8 mg

 MgSO4 * 7 H2O

 125 mg

 (NH4)2SO4

 250 mg

 Ferric ammonium citrate

 25 mg

 Biotin

 0.25 mg

 Calcium pantothenate

 5 mg

 Nicotinic acid

 10 mg

 Pyridoxine

 2 mg

 Thiamine

 5 mg

 Potato extract

 50 ml

 Glucose

 16 g

 Agar

 6 g

Note: This medium does not employ the use of chelated iron,  resulting in an iron-poor medium. The substitution of ferric ammonium  citrate with EDTA-chelated iron is recommended.

Source: Rasmussen, H. Terrestrial orchids from seed to mycotrophic plant. Cambridge University Press, Cambridge. 1995.

 

[OSP] [Technical Data]

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The Orchid Seedbank Project
PO Box 7042
Chandler, AZ 85246