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The Orchid Seedbank Project
PO Box 7042
Chandler, AZ 85246

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Knudson C (Knudson, 1946)

Originally cited by Knudson,1946 (A new nutrient solution for orchid seed germination. American Orchid Society Bulletin, 15, 214-17). 

 Ca(NO3)2 * 4 H2O

 1000 mg

 KH2PO4

 250 mg

 MgSO4 * 7 H2O

 250 mg

 (NH4)2SO4

 500 mg

 FeSO4 * 7 H2O

 25 mg

 MnSO4 * 4 H2O

 7.5 mg

 Sucrose

 20 g

 Agar

 12-15 g

May be supplimented with 100-150 g banana, or 100-200 mL coconut water, and 2 g of vegetable charcoal per liter. 

Note: This medium does not employ the use of chelated iron, resulting  in the formation of insoluble (and therefore unavailable) iron phosphate. As a result, this medium is very iron-poor.

Sources: Arditti, J. Fundamentals of orchid biology. John Wiley and  Sons. 1992.

Rasmussen, H. Terrestrial orchids from seed to mycotrophic plant.  Cambridge University Press, Cambridge. 1995.

 

[OSP] [Technical Data]

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The Orchid Seedbank Project
PO Box 7042
Chandler, AZ 85246