Sections
More Information

The Orchid Seedbank Project
PO Box 7042
Chandler, AZ 85246

knudsonscaglia

Jorge Alejandro Paulete Scaglia's variation on Knudson C

All components listed are in mg/L

Component

Knudson C

Scaglia 1

Scaglia 2

 Ammonium nitrate

  

 500

  

 Ammonium sulfate

 500.0

 500.0

 500.0

 Boric acid

0.056

0.040

0.040

 Calcium nitrate

1000.00

 350.0

 1000.0

 Cupric sulfate * 5 H2O

0.040

  

0.040

 Disodium EDTA

  

 37.00

 37.00

 Ferrous sulfate * 7 H2O

 25.00

 25.0

 25.00

 Magnesium sulfate

 250.00

 250.02

 250.0

 Manganese sulfate * H2O

 7.50

 7.50

 7.50

 Molybdic acid, sodium salt

0.016

0.010

0.016

 Potassium chloride

  

 250.0

  

 Potassium phosphate

 250.00

 250.0

  

 Zinc sulfate * 7 H2O

0.331

0.050

0.300

Organics

  

  

  

 Activated charcoal

  

 2,000

 1,000

 Agar

 12,000

 8,000

 8,000

 Banana powder, 50% malto-dextrin

  

 30,000

 40,000

 Superthrive

  

  

 3 drops

 Dyna-Grow

  

  

 2 cc

 myo-Inositol

  

 50.00

 50.00

 Peptone

  

 2,000

 2,000

 Sucrose

 20,000

 20,000

 20,000

 Thiamine

  

 10.00

 10.00

"There are two formulae as ammonium nitrate and potassium  nitrate are under the Army responsibility and sometimes it is very  difficult to obtain either one."

"Items in blue are salts used by Arditti to modify Knudson C original formula for use in replate."

"150 mL of coconut water may be added to this solution to  increase protocorm multiplication."

Source: Scaglia, pers. communication.

[OSP] [Technical Data]

Questions? Comments?  Email us.
© Copyright 2000 The Orchid Seedbank Project. All rights reserved.

 

The Orchid Seedbank Project
PO Box 7042
Chandler, AZ 85246