Lucke TGZ-L'

Birch sap (see below)  1 liter  Peptone, soya beans, Merck  0.2 g  Yeast extract, Merck  0.1 g  Activated charcoal, powdered  1.5 g  Agar  6 g

Birch sap ("bleeding sap" from Betula spec., harvested when  leaves start to unfold in March or April). Lucke (pers. communication) notes that "the birch sap has to be harvested BEFORE any leaves of the birch trees begin to unfold. Once they begin to unfold no more birch sap will 'bleed' from the trees."

"It is possible to use 250 ml coconut milk and 10 g sugar  instead of birch sap."

A similar media, available commercially, is TGZ-SL. It is sold by:

Manfred Meyer
Eckenheimer Landstr. 334
D - 60435 Frankfurt, tel. +49 69 546552, fax +49 69 5483798.

Source: Lucke, Ehrenfried. Pers. communication.