Mead and Bulard Medium

Originally cited by Mead and Bulard, 1975. Effects of vitamins and nitrogen sources on asymbiotic germination and development of Orchis laxiflora and Ophrys sphegodes. New Phytologist, 74, 33-40. 

CaH4(PO4)2 * H2O  100 mg  CaSO4 * 2 H2O  80 mg  KH2PO4  270 mg  MgSO4 * 7 H2O  240 mg  NH4NO3  80 mg  AlCl3  0.03 mg  CuSO4 * 5 H2O  0.03 mg  FeNa2EDTA  35 mg  H3BO3  1 mg  KI  0.01 mg  MnSO4 * H2O  0.1 mg  NiCl2 * 6 H2O  0.03 mg  ZnSO4 * 7 H2O  1 mg  Casein hydrolysate  5 g  Sucrose  5-80 g  Agar  8 g

Casein hydrolysate can be replaced by the following:

Arginine  100 mg  Aspartic acid  250 mg  Glutamic acid  750 mg  Glycine  50 mg  Histidine  75 mg  Isoleucine  200 mg  Leucine  250 mg  Lysine  250 mg  Methionine  50 mg  Phenylalanine  100 mg  Threonine  100 mg  Tyrosine  50 mg  Valine  200 mg

Source: Rasmussen, H. Terrestrial orchids from seed to mycotrophic plant. Cambridge University Press, Cambridge. 1995.