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The Orchid Seedbank Project
PO Box 7042
Chandler, AZ 85246

melinnorkins

Melin-Norkrans, Modified

Cited by O'Neill, J., for use in germination and culture of  "stubborn" terrestrial species via symbiotic technique. He  notes that this medium is pretty lean, but may be of use for culturing  species that are otherwise very difficult to grow, via the symbiotic technique or otherwise.

 CaCl2

 0.050 g

 NaCl

 0.025 g

 KH2PO4

 0.500 g

 (NH4)2HPO4

 0.250 g

 MgSO4

 0.150 g

 FeCl2 (1% solution)

 1.2 mL

 Glucose

 10 g

 Agar

 10 g

Add to this:

Thiamine HCl: 2 ml of stock solution (stock solution 12.5 mg thiamine  in 250 ml distilled H2O). This has to be sterile filtered  and added to the media after it is autoclaved. (Note: Other formulae utilize thiamine at 10 mg/L concentration, autoclaved while in solution; thiamine decomposes while being autoclaved, but some will survive, and the concentration needs not be very high.)

There are lots of variations on this including one cited in  "Working with Mycorrhizas in Forestry and Agriculture," 1996 pg224 by Brundrett, Bougher, Dell, Grove and Malajczuk in which  the following is substituted:

    iron is supplied as Fe EDTA 20 mg/L (seems to make more sense than FeCl3)
    malt extract 3 g/L
    Thiamine HCl .1 ug/L

(NOTE: The 0.1 ug/L of thiamine is very, very low, and is more likely  to be 0.1 mg/L, although ti could probably be as high as 1 to 10 mg/L). 

Source: O'Neill, J. Personal communication.

 

[OSP] [Technical Data]

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The Orchid Seedbank Project
PO Box 7042
Chandler, AZ 85246