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Melin-Norkrans, Modified
Cited by O'Neill, J., for use in germination and culture of "stubborn" terrestrial species via symbiotic technique. He notes that this medium is pretty lean, but may be of use for culturing species that are otherwise very difficult to grow, via the symbiotic technique or otherwise.
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CaCl2
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0.050 g
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NaCl
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0.025 g
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KH2PO4
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0.500 g
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(NH4)2HPO4
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0.250 g
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MgSO4
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0.150 g
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FeCl2 (1% solution)
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1.2 mL
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Glucose
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10 g
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Agar
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10 g
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Add to this:
Thiamine HCl: 2 ml of stock solution (stock solution 12.5 mg thiamine in 250 ml distilled H2O). This has to be sterile filtered and added to the media after it is autoclaved. (Note: Other formulae utilize thiamine at 10 mg/L concentration, autoclaved while in solution; thiamine decomposes while being autoclaved, but some will survive, and the concentration needs not be very high.)
There are lots of variations on this including one cited in "Working with Mycorrhizas in Forestry and Agriculture," 1996 pg224 by Brundrett, Bougher, Dell, Grove and Malajczuk in which the following is substituted:
iron is supplied as Fe EDTA 20 mg/L (seems to make more sense than FeCl3) malt extract 3 g/L Thiamine HCl .1 ug/L
(NOTE: The 0.1 ug/L of thiamine is very, very low, and is more likely to be 0.1 mg/L, although ti could probably be as high as 1 to 10 mg/L).
Source: O'Neill, J. Personal communication.
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