Murashige and Skoog (1962)

Originally cited by Murashige and Skoog, 1962.

	Thompson notes that while this formula was originally  designed for tissue cultures of tobacco, it may be used for several  species of orchids. This media contains high levels of salts  (nitrogen and potassium are very high) and most of the growth  regulators are not essential for most orchids; the range is given for experimental use.

P-668, P-6668, P-1056, and P-748 are very similar to half-strength M&S medium. 

KH2PO4  170 mg  MgSO4 * 7 H2O  370 mg  NH4NO3  1650 mg  KNO3  1900 mg  CaCl2 * 6 H2O  440 mg  H3BO3  6.2 mg  MnSO4 * 4 H2O  22.3 mg  ZnSO4 * 7 H2O  8.6 mg  KI  0.83 mg  Na2MoO4 * 6 H2O  0.25 mg  CuSO4 * 5 H2O  0.025 mg  CoCl2 * 6 H2O  0.025 mg  FeSO4 * 7 H2O  27.8 mg  Disodium EDTA  37.3 mg  Agar  10 g  Sucrose  30 g  Glycine  2.0 mg  Indoleacetic acid  1 to 30 mg  Kinetin  0.04 to 10 mg Myo-inositol  0.5 mg  Pyridoxine HCl  0.5 mg  Thiamine HCl  0.1 mg

Source: Thompson, P.A. Orchids from Seed. Royal Botanic Gardens, Kew. 1974.