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Sigma P-1056
Same as PhytoTech O-156
These media are provided from their respective suppliers without gelling agent.
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Ammonium nitrate
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825.0 mg
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Boric acid
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3.1 mg
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Calcium chloride, anhydrous
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166.0 mg
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Cobalt chloride * 6 H2O
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0.0125 mg
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Cupric sulfate * 5 H2O
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0.0125 mg
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Na2 * EDTA
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37.3 mg
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Ferrous sulfate * 7 H2O
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27.85 mg
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Magnesium sulfate
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90.35 mg
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Manganese sulfate * H2O
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8.45 mg
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Molybdic acid, sodium salt * H2O
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0.125 mg
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Potassium iodide
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0.415 mg
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Potassium nitrate
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950.0 mg
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Potassium phosphate monobasic
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85.0 mg
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Zinc sulfate * 7 H2O
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5.3 mg
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Activated charcoal
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2000.0 mg
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MES, free acid (buffer)
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1000.0 mg
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Myo-inositol
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100.0 mg
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Thiamine (Vitamin B1)
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10.0 mg
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Nicotinic acid (free acid) (Vitamin B3)
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1.0 mg
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Pyridoxine HCl (Vitamin B6)
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1.0 mg
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Peptone
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2000.0 mg
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Sucrose
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20,000.0 mg
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Banana powder (50% malto-dextrin)
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30,000.0 mg
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Total weight for 1 liter of mix: 57.31 grams
Note: This medium is excellent for replate of epiphytic orchids. It does tend to cause proliferation of protocorms when used at full strength; as a result, it may be used to multiply the number of plantlets from protocorms, or it may be diluted to 90%, 80%, or even lower concentrations for general plantlet culture. It is not recommended at full strength for slow-growing plants, or those that do not do well with "hot" media.
Source: PhytoTechnology Labs catalog. Reprinted with permission.
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