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Tashima Medium
Originally cited by Tashima et al., 1978. In vitro development from seed to flower in Gastrodia verrucosa under fungal symbiosis. Transactions of the mycological society of Japan, 19, 449-53.
For symbiotic culture of Gastrodia verrucosa.
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Ca(NO3)2 * 4 H2O
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117.0 mg
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KH2PO4
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38.3 mg
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MgSO4 * 7 H2O
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120.0 mg
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NH4NO3
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57.5 mg
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CuSO4 * 5 H2O
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0.05 mg
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H3BO3
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0.6 mg
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H2MoO4 * H2O
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0.02 mg
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MnCl2 * 4 H2O
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0.4 mg
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ZnSO4 * 7 H2O
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0.05 mg
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Ferric citrate
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10 mg
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Watermelon juice
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70 ml
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Sucrose
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20 g
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Meal or withered bamboo leaves*
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Adjust pH to 5.4-5.5 after autoclaving.
* Leaves rinsed in tap water, boiled at 100 C for 60 minutes in DI, dried at 80 C with hot air, and finely ground. Serves as carbon source for fungus, and substrate (replacing agar). Quantity not specified.
Source: Rasmussen, H. Terrestrial orchids from seed to mycotrophic plant. Cambridge University Press, Cambridge. 1995.
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