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Vacin and Went Modified Basal Salt Medium
PhytoTechnology V 891. Modified from Vacin and Went (1949) through the addition of ferrous sulfate + EDTA.
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Calcium phosphate, tribasic [Ca3(PO4)2]
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200 mg
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Potassium phosphate, monobasic [KH2PO4]
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250 mg
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Potassium nitrate [KNO3]
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525 mg
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Magnesium sulfate [MgSO4]
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122.1 mg
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Ammonium sulfate [(NH4)2SO4]
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500 mg
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Na2EDTA
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37.26 mg
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Ferrous sulfate * 7 H2O
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27.8 mg
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Manganese sulfate [MnSO4 * H2O]
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5.0875 mg
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Sucrose
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20,000 mg
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Total weight: 21.667 grams per liter.
Note: V 895 from PhytoTech is identical, but has 8,000 mg of agar added per liter of media.
Source: PhytoTechnology Labs catalog. Reprinted with permission.
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